Part:BBa_K2805010:Design

{pPGK1-HY1}-{pTEF1-PcyA}

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal suffix found in sequence at 1740
  Illegal BglII site found at 885
  Illegal BglII site found at 3310
  Illegal BamHI site found at 1460
  Illegal XhoI site found at 1569
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2863
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The primary concern was Dueber MoClo and GoldenGate compatibility. These sequences were designed to be robustly assembled and integrated into the S. cerevisiae genome.

Source

Cyanobacteria (PcyA) and Arabidopsis thaliana (PcyA)

References

1. Benzinger D, Khammash M. Pulsatile inputs achieve tunable attenuation of gene expression variability and graded multi-gene regulation. Nature Communications. 2018;9(1):3521. https://doi.org/10.1038/s41467-018-05882-2. doi: 10.1038/s41467-018-05882-2.

2. Hochrein L, Machens F, Messerschmidt K, Mueller-Roeber B. PhiReX: A programmable and red light-regulated protein expression switch for yeast. Nucleic Acids Res. 2017;45(15):9193-9205. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5587811/. doi: 10.1093/nar/gkx610.

3. Lee ME, DeLoache WC, Cervantes B, Dueber JE. A highly characterized yeast toolkit for modular, multipart assembly. ACS Synth Biol. 2015;4(9):975-986. https://doi.org/10.1021/sb500366v. doi: 10.1021/sb500366v.